Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803

Stefanie Böhmer, Katharina Köninger, Alvaro Gomez-Baraibar, Samiro Bojarra, Carolin Mügge, Sandy Schmidt, Marc Nowaczyk, Robert Kourist

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Photosynthetic water-splitting is a powerful force to drive selective redox reactions. The need of highly expensive redox partners such as NADPH and their regeneration is one of the main bottlenecks for the application of biocatalysis at an industrial scale. Recently, the possibility of using the photosystem of cyanobacteria to supply high amounts of reduced nicotinamide to a recombinant enoate reductase opened a new strategy for overcoming this hurdle. This paper presents the expansion of the photosynthetic regeneration system to a Baeyer–Villiger monooxygenase. Despite the potential of this strategy, this work also presents some of the encountered challenges as well as possible solutions, which will require further investigation. The successful enzymatic oxygenation shows that cyanobacterial whole-cell biocatalysis is an applicable approach that allows fuelling selective oxyfunctionalisation reactions at the expense of light and water. Yet, several hurdles such as side-reactions and the cell-density limitation, probably due to self-shading of the cells, will have to be overcome on the way to synthetic applications.
Originalspracheenglisch
Aufsatznummer240
Seitenumfang8
FachzeitschriftCatalysts
Jahrgang7
Ausgabenummer8
DOIs
PublikationsstatusVeröffentlicht - Aug 2017

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water splitting
regeneration
Fueling
Oxygenation
Niacinamide
Water
Redox reactions
Mixed Function Oxygenases
cells
NADP
nicotinamide
Oxidoreductases
refueling
oxygenation
expansion
water
Biocatalysis
Cyanobacteria
Oxidation-Reduction

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Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803. / Böhmer, Stefanie; Köninger, Katharina; Gomez-Baraibar, Alvaro; Bojarra, Samiro; Mügge, Carolin; Schmidt, Sandy; Nowaczyk, Marc; Kourist, Robert.

in: Catalysts, Jahrgang 7, Nr. 8, 240, 08.2017.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Böhmer, Stefanie ; Köninger, Katharina ; Gomez-Baraibar, Alvaro ; Bojarra, Samiro ; Mügge, Carolin ; Schmidt, Sandy ; Nowaczyk, Marc ; Kourist, Robert. / Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803. in: Catalysts. 2017 ; Jahrgang 7, Nr. 8.
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