Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH

Gernot Strohmeier, Inge Eiteljörg, Anna Schwarz, Margit Winkler

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

The direct generation of aldehydes from carboxylic acids is often a challenging synthetic task but undoubtedly attractive in view of abundant supply of such feedstocks from nature. Though long known, biocatalytic carboxylate reductions are at an early stage of development, presumably because of their co-factor requirement. To establish an alternative to whole-cell-based carboxylate reductions which are limited by side reactions, we developed an in vitro multi-enzyme system that allows for quantitative reductions of various carboxylic acids with full recycling of all cofactors and prevention of undesired over-reductions. Regeneration of adenosine 5′-triphosphate is achieved through the simultaneous action of polyphosphate kinases from Meiothermus ruber and Sinorhizobium meliloti and β-nicotinamide adenine dinucleotide 2′-phosphate is reduced by a glucose dehydrogenase. Under these conditions and in the presence of the carboxylate reductases from Neurospora crassa or Nocardia iowensis, various aromatic, heterocyclic and aliphatic carboxylic acids were quantitatively reduced to the respective aldehydes.

Originalspracheenglisch
Seiten (von - bis)6119-6123
Seitenumfang5
FachzeitschriftChemistry - A European Journal
Jahrgang25
Ausgabenummer24
DOIs
PublikationsstatusVeröffentlicht - 26 Apr 2019

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Adenosinetriphosphate
Aldehydes
NADP
Carboxylic Acids
Adenosine Triphosphate
Carboxylic acids
Glucose 1-Dehydrogenase
NAD
Adenosine
Feedstocks
Glucose
Recycling
Oxidoreductases
Phosphates
Enzymes

Schlagwörter

    ASJC Scopus subject areas

    • !!Catalysis
    • Organische Chemie

    Fields of Expertise

    • Human- & Biotechnology

    Dies zitieren

    Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH. / Strohmeier, Gernot; Eiteljörg, Inge; Schwarz, Anna; Winkler, Margit.

    in: Chemistry - A European Journal, Jahrgang 25, Nr. 24, 26.04.2019, S. 6119-6123.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

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    AU - Schwarz, Anna

    AU - Winkler, Margit

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