Enhancing the sensitivity of DNA microarray using dye-doped silica nanoparticles: Detection of human papilloma virus

F. Enrichi, R. Riccò, A. Meneghello, R. Pierobon, G. Canton, E. Cretaio

Publikation: Beitrag in Buch/Bericht/KonferenzbandBeitrag in einem KonferenzbandForschungBegutachtung

Abstract

DNA microarray is a high-throughput technology used for detection and quantification of nucleic acid molecules and others of biological interest. The analysis is based on the specific hybridization between probe sequences deposited in array and a target ss-DNA amplified by PCR and functionalized by a fluorescent dye. Organic labels have well known disadvantages like photobleaching and low signal intensities, which put a limitation to the lower amount of DNA material that can be detected. Therefore for trace analysis the development of more efficient biomarkers is required. With this aim we present in this paper the synthesis and application of alternative hybrid nanosystems obtained by incorporating standard fluorescent molecules into monodisperse silica nanoparticles. Efficient application to the detection of Human Papilloma Virus is demonstrated. This virus is associated to the formation of cervical cancer, a leading cause of death by cancer for women worldwide. It is shown that the use of the novel biomarkers increases the optical signal of about one order of magnitude with respect to the free dyes or quantum dots in conventional instruments. This is due to the high number of molecules that can be accommodated into each nanoparticle, to the reduced photobleaching and to the improved environmental protection of the dyes when encapsulated in the silica matrix. The cheap and easy synthesis of these luminescent particles, the stability in water, the surface functionalizability and bio-compatibility make them very promising for present and future bio-labeling and bio-imaging applications.

Originalspracheenglisch
TitelAIP Conference Proceedings
Seiten154-157
Seitenumfang4
Band1275
DOIs
PublikationsstatusVeröffentlicht - 2010
Extern publiziertJa
VeranstaltungBONSAI Project Symposium: Breakthroughs in Nanoparticles for Bio-Imaging - Rome, Italien
Dauer: 8 Apr 20109 Apr 2010

Konferenz

KonferenzBONSAI Project Symposium: Breakthroughs in Nanoparticles for Bio-Imaging
LandItalien
OrtRome
Zeitraum8/04/109/04/10

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    ASJC Scopus subject areas

    • !!Physics and Astronomy(all)

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    Enrichi, F., Riccò, R., Meneghello, A., Pierobon, R., Canton, G., & Cretaio, E. (2010). Enhancing the sensitivity of DNA microarray using dye-doped silica nanoparticles: Detection of human papilloma virus. in AIP Conference Proceedings (Band 1275, S. 154-157) https://doi.org/10.1063/1.3505067