Enhanced Activity of Enzymes Encapsulated in Hydrophilic Metal–Organic Frameworks

Weibin Liang, Huoshu Xu, Francesco Carraro, Natasha K. Maddigan, Qiaowei Li, Stephen G. Bell, David M. Huang, Andrew Tarzia, Marcello B. Solomon, Heinz Amenitsch, Lisa Vaccari, Christopher J. Sumby, Paolo Falcaro, Christian J. Doonan

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Encapsulation of biomacromolecules in metal–organic frameworks (MOFs) can preserve biological functionality in harsh environments. Despite the success of this approach, termed biomimietic mineralization, limited consideration has been given to the chemistry of the MOF coating. Here we show that enzymes encapsulated within hydrophilic MAF-7 or ZIF-90 retain enzymatic activity upon encapsulation and when exposed to high temperatures, denaturing or proteolytic agents, and organic solvents, whereas hydrophobic ZIF-8 affords inactive catalase and negligible protection to urease.
Spracheenglisch
Seiten2348-2355
FachzeitschriftJournal of the American Chemical Society
Jahrgang141
Ausgabennummer6
DOIs
StatusVeröffentlicht - 12 Jan 2019

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Encapsulation
Enzymes
Metals
Organic Chemistry
Metal coatings
Organic coatings
Urease
Catalase
Organic solvents
Temperature

Schlagwörter

    ASJC Scopus subject areas

    • !!Chemistry(all)
    • !!Biochemistry
    • !!Catalysis
    • !!Colloid and Surface Chemistry

    Fields of Expertise

    • Advanced Materials Science

    Dies zitieren

    Enhanced Activity of Enzymes Encapsulated in Hydrophilic Metal–Organic Frameworks. / Liang, Weibin; Xu, Huoshu; Carraro, Francesco; Maddigan, Natasha K.; Li, Qiaowei; Bell, Stephen G.; Huang, David M.; Tarzia, Andrew; Solomon, Marcello B.; Amenitsch, Heinz; Vaccari, Lisa; Sumby, Christopher J.; Falcaro, Paolo; Doonan, Christian J.

    in: Journal of the American Chemical Society, Jahrgang 141, Nr. 6, 12.01.2019, S. 2348-2355.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Liang, W, Xu, H, Carraro, F, Maddigan, NK, Li, Q, Bell, SG, Huang, DM, Tarzia, A, Solomon, MB, Amenitsch, H, Vaccari, L, Sumby, CJ, Falcaro, P & Doonan, CJ 2019, 'Enhanced Activity of Enzymes Encapsulated in Hydrophilic Metal–Organic Frameworks', Journal of the American Chemical Society, Jg. 141, Nr. 6, S. 2348-2355. https://doi.org/10.1021/jacs.8b10302
    Liang, Weibin ; Xu, Huoshu ; Carraro, Francesco ; Maddigan, Natasha K. ; Li, Qiaowei ; Bell, Stephen G. ; Huang, David M. ; Tarzia, Andrew ; Solomon, Marcello B. ; Amenitsch, Heinz ; Vaccari, Lisa ; Sumby, Christopher J. ; Falcaro, Paolo ; Doonan, Christian J. / Enhanced Activity of Enzymes Encapsulated in Hydrophilic Metal–Organic Frameworks. in: Journal of the American Chemical Society. 2019 ; Jahrgang 141, Nr. 6. S. 2348-2355.
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