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Abstract
Numerous synthetic biology endeavors require well-tuned co-expression of functional components for success. Classically, monodirectional promoters (MDPs) have been used for such applications, but MDPs are limited in terms of multi-gene co-expression capabilities. Consequently, there is a pressing need for new tools with improved flexibility in terms of genetic circuit design, metabolic pathway assembly, and optimization. Here, motivated by nature's use of bidirectional promoters (BDPs) as a solution for efficient gene co-expression, we generate a library of 168 synthetic BDPs in the yeast Komagataella phaffii (syn. Pichia pastoris), leveraging naturally occurring BDPs as a parts repository. This library of synthetic BDPs allows for rapid screening of diverse expression profiles and ratios to optimize gene co-expression, including for metabolic pathways (taxadiene, β-carotene). The modular design strategies applied for creating the BDP library could be relevant in other eukaryotic hosts, enabling a myriad of metabolic engineering and synthetic biology applications.
Originalsprache | englisch |
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Seiten (von - bis) | 3589 |
Fachzeitschrift | Nature Communications |
Jahrgang | 9 |
Ausgabenummer | 1 |
DOIs | |
Publikationsstatus | Veröffentlicht - 4 Sept. 2018 |
Fields of Expertise
- Human- & Biotechnology
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A3b Industrielle Enzyme durch Gentechnik und Protein Engineering
1/01/95 → …
Projekt: Forschungsprojekt
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DK Molekulare Enzymologie
Luley, C., Egger, S., Griengl, H., Lehsl, A., Lypetska, K., Malik, I. S., Schwab, H., Flis, V. V., Glieder, A., Fuchs, A., Marlingapla Halasiddappa, L., Hermetter, A., Pratter, S., Prattner, J., Freigaßner, M. E., Czabany, T., Schitter-Sollner, S., Macheroux, P., Eixelsberger, T., Thonhauser, M., Horvath, S., Nidetzky, B., Winkler, A., Daum, G., Connerth, M. & Rajakumari, S.
1/01/05 → 31/12/17
Projekt: Forschungsprojekt