TY - JOUR
T1 - Effect of Noncanonical Amino Acids on Protein-Carbohydrate Interactions
T2 - Structure, Dynamics, and Carbohydrate Affinity of a Lectin Engineered with Fluorinated Tryptophan Analogs
AU - Tobola, Felix
AU - Lelimousin, Mickael
AU - Varrot, Annabelle
AU - Gillon, Emilie
AU - Darnhofer, Barbara
AU - Blixt, Ola
AU - Birner-Gruenberger, Ruth
AU - Imberty, Anne
AU - Wiltschi, Birgit
PY - 2018/5/29
Y1 - 2018/5/29
N2 - Protein-carbohydrate interactions play crucial roles in biology. Understanding and modifying these interactions is of major interest for fighting many diseases. We took a synthetic biology approach and incorporated noncanonical amino acids into a bacterial lectin to modulate its interactions with carbohydrates. We focused on tryptophan, which is prevalent in carbohydrate binding sites. The exchange of the tryptophan residues with analogs fluorinated at different positions resulted in three distinctly fluorinated variants of the lectin from Ralstonia solanacearum. We observed differences in stability and affinity toward fucosylated glycans and rationalized them by X-ray and modeling studies. While fluorination decreased the aromaticity of the indole ring and, therefore, the strength of carbohydrate-aromatic interactions, additional weak hydrogen bonds were formed between fluorine and the ligand hydroxyl groups. Our approach opens new possibilities to engineer carbohydrate receptors.
AB - Protein-carbohydrate interactions play crucial roles in biology. Understanding and modifying these interactions is of major interest for fighting many diseases. We took a synthetic biology approach and incorporated noncanonical amino acids into a bacterial lectin to modulate its interactions with carbohydrates. We focused on tryptophan, which is prevalent in carbohydrate binding sites. The exchange of the tryptophan residues with analogs fluorinated at different positions resulted in three distinctly fluorinated variants of the lectin from Ralstonia solanacearum. We observed differences in stability and affinity toward fucosylated glycans and rationalized them by X-ray and modeling studies. While fluorination decreased the aromaticity of the indole ring and, therefore, the strength of carbohydrate-aromatic interactions, additional weak hydrogen bonds were formed between fluorine and the ligand hydroxyl groups. Our approach opens new possibilities to engineer carbohydrate receptors.
UR - http://www.scopus.com/inward/record.url?scp=85048004150&partnerID=8YFLogxK
U2 - 10.1021/acschembio.8b00377
DO - 10.1021/acschembio.8b00377
M3 - Article
AN - SCOPUS:85048004150
SN - 1554-8929
VL - 13
SP - 2211
EP - 2219
JO - ACS Chemical Biology
JF - ACS Chemical Biology
IS - 8
ER -