Development of a molecular approach to describe the composition of Trichoderma communities

R. Meincke; N. Weinert; V. Radl; M. Schloter; K. Smalla; Gabriele Berg

doi:https://doi.org/10.1016/j.mimet.2009.11.001

Development of a molecular approach to describe the composition of Trichoderma communities

R. Meincke, N. Weinert, V. Radl, M. Schloter, K. Smalla, Gabriele Berg

Institut für Umweltbiotechnologie (6530)

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

Abstract

Trichoderma and its teleomorphic stage Hypocrea play a key role for ecosystem functioning in terrestrial habitats. However, little is known about the ecology of the fungus. In this study we developed a novel Trichoderma-specific primer pair for diversity analysis. Based on a broad range master alignment, specific Trichoderma primers (ITSTrF/ITSTrR) were designed that comprise an approximate 650 bp fragment of the internal transcribed spacer region from all taxonomic clades of the genus Trichoderma. This amplicon is suitable for identification with TrichoKey and TrichoBLAST. Moreover, this primer system was successfully applied to study the Trichoderma communities in the rhizosphere of different potato genotypes grown at two field sites in Germany. Cloning and sequencing confirmed the specificity of the primer and revealed a site-dependent Trichoderma composition. Based on the new primer system a semi …

Originalsprache	englisch
Seiten (von - bis)	63-69
Fachzeitschrift	Journal of microbiological methods
Jahrgang	80
DOIs	https://doi.org/10.1016/j.mimet.2009.11.001
Publikationsstatus	Veröffentlicht - 2009

Treatment code (Nähere Zuordnung)

Basic - Fundamental (Grundlagenforschung)

UN SDGs

Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung

Zugriff auf Dokument

https://doi.org/10.1016/j.mimet.2009.11.001

Dieses zitieren

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abstract = "Trichoderma and its teleomorphic stage Hypocrea play a key role for ecosystem functioning in terrestrial habitats. However, little is known about the ecology of the fungus. In this study we developed a novel Trichoderma-specific primer pair for diversity analysis. Based on a broad range master alignment, specific Trichoderma primers (ITSTrF/ITSTrR) were designed that comprise an approximate 650 bp fragment of the internal transcribed spacer region from all taxonomic clades of the genus Trichoderma. This amplicon is suitable for identification with TrichoKey and TrichoBLAST. Moreover, this primer system was successfully applied to study the Trichoderma communities in the rhizosphere of different potato genotypes grown at two field sites in Germany. Cloning and sequencing confirmed the specificity of the primer and revealed a site-dependent Trichoderma composition. Based on the new primer system a semi …",

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AU - Meincke, R.

AU - Weinert, N.

AU - Radl, V.

AU - Schloter, M.

AU - Smalla, K.

AU - Berg, Gabriele

PY - 2009

Y1 - 2009

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DO - https://doi.org/10.1016/j.mimet.2009.11.001

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