Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Publikation: Beitrag in einer FachzeitschriftArtikelBegutachtung

Abstract

Class VIII self-sufficient CYP from Aspergillus fumigatus (CYP505X) and a variant with five mutations were heterologously expressed in Pichia pastoris. Ibuprofen – a widely used non-steroidal anti-inflammatory drug (NSAID) – was oxidized most efficiently in a whole cell mediated biotransformation. The two major metabolites were isolated by preparative HPLC and their structure elucidated by NMR. Specifically, ibuprofen was hydroxylated at the tertiary carbon atom and the benzylic position on the lipophilic side chain.

Originalspracheenglisch
Seiten (von - bis)525-528
Seitenumfang4
FachzeitschriftBiocatalysis and Agricultural Biotechnology
Jahrgang17
DOIs
PublikationsstatusVeröffentlicht - 2019

ASJC Scopus subject areas

  • Angewandte Mikrobiologie und Biotechnologie
  • Bioengineering
  • Lebensmittelwissenschaften
  • Agronomie und Nutzpflanzenwissenschaften
  • Biotechnology

Fields of Expertise

  • Human- & Biotechnology

Fingerprint

Untersuchen Sie die Forschungsthemen von „Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren