Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Class VIII self-sufficient CYP from Aspergillus fumigatus (CYP505X) and a variant with five mutations were heterologously expressed in Pichia pastoris. Ibuprofen – a widely used non-steroidal anti-inflammatory drug (NSAID) – was oxidized most efficiently in a whole cell mediated biotransformation. The two major metabolites were isolated by preparative HPLC and their structure elucidated by NMR. Specifically, ibuprofen was hydroxylated at the tertiary carbon atom and the benzylic position on the lipophilic side chain.

Originalspracheenglisch
Seiten (von - bis)525-528
Seitenumfang4
FachzeitschriftBiocatalysis and Agricultural Biotechnology
Jahrgang17
DOIs
PublikationsstatusVeröffentlicht - 2019

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ibuprofen
Hydroxylation
Pichia pastoris
Biocatalysts
Pichia
Aspergillus
Ibuprofen
hydroxylation
Metabolites
cytochrome P-450
Cytochrome P-450 Enzyme System
Nuclear magnetic resonance
Atoms
Carbon
Aspergillus fumigatus
nonsteroidal anti-inflammatory agents
Enzymes
Biotransformation
biotransformation
Anti-Inflammatory Agents

Schlagwörter

    ASJC Scopus subject areas

    • !!Applied Microbiology and Biotechnology
    • Bioengineering
    • !!Food Science
    • !!Agronomy and Crop Science
    • Biotechnology

    Fields of Expertise

    • Human- & Biotechnology

    Dies zitieren

    Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst. / Rinnofner, Claudia; Kerschbaumer, Bianca; Weber, Hansjörg; Glieder, Anton; Winkler, Margit.

    in: Biocatalysis and Agricultural Biotechnology, Jahrgang 17, 2019, S. 525-528.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

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    AU - Rinnofner, Claudia

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    AU - Weber, Hansjörg

    AU - Glieder, Anton

    AU - Winkler, Margit

    PY - 2019

    Y1 - 2019

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    KW - API Metabolism

    KW - Cytochrome P450

    KW - Hydroxylation

    KW - Ibuprofen

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    JO - Biocatalysis and Agricultural Biotechnology

    JF - Biocatalysis and Agricultural Biotechnology

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