Cyanobacteria Biorefinery — Production of poly(3-hydroxybutyrate) with Synechocystis salina and utilisation of residual biomass

K. Meixner, A. Kovalcik, E. Sykacek, M. Gruber-Brunhumer, W. Zeilinger, K. Markl, C. Haas, I. Fritz, N. Mundigler, F. Stelzer, M. Neureiter, W. Fuchs, B. Drosg

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

This study evaluates a biorefinery concept for producing poly(3-hydroxybutyrate) (PHB) with the cyanobacterial strain Synechocystis salina. Due to this reason, pigment extraction and cell disruption were investigated as pre-treatment steps for the harvested cyanobacterial biomass. The results demonstrated that at least pigment removal was necessary to obtain PHB with processable quality (weight average molecular weight: 569–988 kg mol‐1, melting temperature: 177–182 °C), which was comparable to heterotrophically produced PHB. The removed pigments could be utilised as additional by-products (chlorophylls 0.27–1.98 mg g‐1 TS, carotenoids 0.21–1.51 mg g‐1 TS, phycocyanin 0–127 mg g‐1 TS), whose concentration depended on the used nutrient source. Since the residual biomass still contained proteins (242 mg g‐1 TS), carbohydrates (6.1 mg g‐1 TS) and lipids (14 mg g‐1 TS), it could be used as animal feed or converted to biomethane (348 mn 3 t‐1 VS) and fertiliser. The obtained results indicate that the combination of photoautotrophic PHB production with pigment extraction and utilisation of residual biomass offer the highest potential, since it contributes to decrease the environmental footprint of the process and because biomass could be used in a cascading way and the nutrient cycle could be closed.

Originalspracheenglisch
Seiten (von - bis)46-53
Seitenumfang8
FachzeitschriftJournal of Biotechnology
Jahrgang265
DOIs
PublikationsstatusVeröffentlicht - 10 Jan 2018

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Synechocystis
Cyanobacteria
Pigments
Biomass
Nutrients
Phycocyanin
Food
Fertilizers
Chlorophyll
Carotenoids
Carbohydrates
Lipids
Freezing
Byproducts
Melting point
Animals
Molecular Weight
Molecular weight
Proteins
Weights and Measures

Schlagwörter

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • !!Applied Microbiology and Biotechnology

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    Cyanobacteria Biorefinery — Production of poly(3-hydroxybutyrate) with Synechocystis salina and utilisation of residual biomass. / Meixner, K.; Kovalcik, A.; Sykacek, E.; Gruber-Brunhumer, M.; Zeilinger, W.; Markl, K.; Haas, C.; Fritz, I.; Mundigler, N.; Stelzer, F.; Neureiter, M.; Fuchs, W.; Drosg, B.

    in: Journal of Biotechnology, Jahrgang 265, 10.01.2018, S. 46-53.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Meixner, K, Kovalcik, A, Sykacek, E, Gruber-Brunhumer, M, Zeilinger, W, Markl, K, Haas, C, Fritz, I, Mundigler, N, Stelzer, F, Neureiter, M, Fuchs, W & Drosg, B 2018, 'Cyanobacteria Biorefinery — Production of poly(3-hydroxybutyrate) with Synechocystis salina and utilisation of residual biomass' Journal of Biotechnology, Jg. 265, S. 46-53. https://doi.org/10.1016/j.jbiotec.2017.10.020
    Meixner, K. ; Kovalcik, A. ; Sykacek, E. ; Gruber-Brunhumer, M. ; Zeilinger, W. ; Markl, K. ; Haas, C. ; Fritz, I. ; Mundigler, N. ; Stelzer, F. ; Neureiter, M. ; Fuchs, W. ; Drosg, B. / Cyanobacteria Biorefinery — Production of poly(3-hydroxybutyrate) with Synechocystis salina and utilisation of residual biomass. in: Journal of Biotechnology. 2018 ; Jahrgang 265. S. 46-53.
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    abstract = "This study evaluates a biorefinery concept for producing poly(3-hydroxybutyrate) (PHB) with the cyanobacterial strain Synechocystis salina. Due to this reason, pigment extraction and cell disruption were investigated as pre-treatment steps for the harvested cyanobacterial biomass. The results demonstrated that at least pigment removal was necessary to obtain PHB with processable quality (weight average molecular weight: 569–988 kg mol‐1, melting temperature: 177–182 °C), which was comparable to heterotrophically produced PHB. The removed pigments could be utilised as additional by-products (chlorophylls 0.27–1.98 mg g‐1 TS, carotenoids 0.21–1.51 mg g‐1 TS, phycocyanin 0–127 mg g‐1 TS), whose concentration depended on the used nutrient source. Since the residual biomass still contained proteins (242 mg g‐1 TS), carbohydrates (6.1 mg g‐1 TS) and lipids (14 mg g‐1 TS), it could be used as animal feed or converted to biomethane (348 mn 3 t‐1 VS) and fertiliser. The obtained results indicate that the combination of photoautotrophic PHB production with pigment extraction and utilisation of residual biomass offer the highest potential, since it contributes to decrease the environmental footprint of the process and because biomass could be used in a cascading way and the nutrient cycle could be closed.",
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    AU - Meixner, K.

    AU - Kovalcik, A.

    AU - Sykacek, E.

    AU - Gruber-Brunhumer, M.

    AU - Zeilinger, W.

    AU - Markl, K.

    AU - Haas, C.

    AU - Fritz, I.

    AU - Mundigler, N.

    AU - Stelzer, F.

    AU - Neureiter, M.

    AU - Fuchs, W.

    AU - Drosg, B.

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    AB - This study evaluates a biorefinery concept for producing poly(3-hydroxybutyrate) (PHB) with the cyanobacterial strain Synechocystis salina. Due to this reason, pigment extraction and cell disruption were investigated as pre-treatment steps for the harvested cyanobacterial biomass. The results demonstrated that at least pigment removal was necessary to obtain PHB with processable quality (weight average molecular weight: 569–988 kg mol‐1, melting temperature: 177–182 °C), which was comparable to heterotrophically produced PHB. The removed pigments could be utilised as additional by-products (chlorophylls 0.27–1.98 mg g‐1 TS, carotenoids 0.21–1.51 mg g‐1 TS, phycocyanin 0–127 mg g‐1 TS), whose concentration depended on the used nutrient source. Since the residual biomass still contained proteins (242 mg g‐1 TS), carbohydrates (6.1 mg g‐1 TS) and lipids (14 mg g‐1 TS), it could be used as animal feed or converted to biomethane (348 mn 3 t‐1 VS) and fertiliser. The obtained results indicate that the combination of photoautotrophic PHB production with pigment extraction and utilisation of residual biomass offer the highest potential, since it contributes to decrease the environmental footprint of the process and because biomass could be used in a cascading way and the nutrient cycle could be closed.

    KW - Anaerobic digestion

    KW - Downstream processing

    KW - Molecular weight

    KW - Pigments

    KW - Residual biomass

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    JO - Journal of Biotechnology

    JF - Journal of Biotechnology

    SN - 0168-1656

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