TY - JOUR
T1 - Comparisons of diazotrophic communities in native and agricultural desert ecosystems reveal plants as important drivers in diversity
AU - Köberl, Martina
AU - Erlacher, Armin
AU - Ramadan, Elshahat M.
AU - El-Arabi, Tarek F.
AU - Müller, Henry
AU - Bragina, Anastasia
AU - Berg, Gabriele
PY - 2016/2
Y1 - 2016/2
N2 - Diazotrophs provide the only biological source of fixed atmospheric nitrogen in the biosphere. Although they are the key player for plant-available nitrogen, less is known about their diversity and potential importance in arid ecosystems. We investigated the nitrogenase gene diversity in native and agricultural desert soil as well as within root-associated microbiota of medicinal plants grown in Egypt through the combination of nifH-specific qPCR, fingerprints, amplicon pyrosequencing and fluorescence in situ hybridization–confocal laser scanning microscopy. Although the diazotrophic microbiota were characterized by generally high abundances and diversity, statistically significant differences were found between both soils, the different microhabitats, and between the investigated plants (Matricaria chamomilla L., Calendula officinalis L. and Solanum distichum Schumach. and Thonn.). We observed a considerable …
AB - Diazotrophs provide the only biological source of fixed atmospheric nitrogen in the biosphere. Although they are the key player for plant-available nitrogen, less is known about their diversity and potential importance in arid ecosystems. We investigated the nitrogenase gene diversity in native and agricultural desert soil as well as within root-associated microbiota of medicinal plants grown in Egypt through the combination of nifH-specific qPCR, fingerprints, amplicon pyrosequencing and fluorescence in situ hybridization–confocal laser scanning microscopy. Although the diazotrophic microbiota were characterized by generally high abundances and diversity, statistically significant differences were found between both soils, the different microhabitats, and between the investigated plants (Matricaria chamomilla L., Calendula officinalis L. and Solanum distichum Schumach. and Thonn.). We observed a considerable …
UR - http://femsec.oxfordjournals.org/content/92/2/fiv166
U2 - 10.1093/femsec/fiv166
DO - 10.1093/femsec/fiv166
M3 - Article
SN - 1574-6941
VL - 92
JO - FEMS Microbiology Ecology
JF - FEMS Microbiology Ecology
IS - 2
M1 - fiv166
ER -