TY - JOUR
T1 - Comparative proteomic study of phytotoxic effects of silver nanoparticles and silver ions on tobacco plants
AU - Peharec Štefanić, Petra
AU - Jarnević, Martina
AU - Cvjetko, Petra
AU - Biba, Renata
AU - Šikić, Sandra
AU - Tkalec, Mirta
AU - Cindrić, Mario
AU - Letofsky-Papst, Ilse
AU - Balen, Biljana
PY - 2019/8/1
Y1 - 2019/8/1
N2 - Widespread application of silver nanoparticles (AgNPs), due to their antibacterial and antifungal properties, increases their release into the environment and potential detrimental impact on living organisms. Plants may serve as a potential pathway for AgNPs bioaccumulation and a route into the food chain, hence investigation of AgNP phytotoxic effects are of particular importance. Since proteins are directly involved in stress response, studies of their abundance changes can help elucidate the mechanism of the AgNP-mediated phytotoxicity. In this study, we investigated proteomic changes in tobacco (Nicotiana tabacum) exposed to AgNPs and ionic silver (AgNO3). A high overlap of differently abundant proteins was found in root after exposure to both treatments, while in leaf, almost a half of the proteins exhibited different abundance level between treatments, indicating tissue-specific responses. Majority of the identified proteins were down-regulated in both tissues after exposure to either AgNPs or AgNO3; in roots, the most affected proteins were those involved in response to abiotic and biotic stimuli and oxidative stress, while in leaf, both treatments had the most prominent effect on photosynthesis-related proteins. However, since AgNPs induced higher suppression of protein abundance than AgNO3, we conclude that AgNP effects can, at least partially, be attributed to nanoparticle form.
AB - Widespread application of silver nanoparticles (AgNPs), due to their antibacterial and antifungal properties, increases their release into the environment and potential detrimental impact on living organisms. Plants may serve as a potential pathway for AgNPs bioaccumulation and a route into the food chain, hence investigation of AgNP phytotoxic effects are of particular importance. Since proteins are directly involved in stress response, studies of their abundance changes can help elucidate the mechanism of the AgNP-mediated phytotoxicity. In this study, we investigated proteomic changes in tobacco (Nicotiana tabacum) exposed to AgNPs and ionic silver (AgNO3). A high overlap of differently abundant proteins was found in root after exposure to both treatments, while in leaf, almost a half of the proteins exhibited different abundance level between treatments, indicating tissue-specific responses. Majority of the identified proteins were down-regulated in both tissues after exposure to either AgNPs or AgNO3; in roots, the most affected proteins were those involved in response to abiotic and biotic stimuli and oxidative stress, while in leaf, both treatments had the most prominent effect on photosynthesis-related proteins. However, since AgNPs induced higher suppression of protein abundance than AgNO3, we conclude that AgNP effects can, at least partially, be attributed to nanoparticle form.
KW - Nicotiana tabacum
KW - Phytotoxicity
KW - Proteomics
KW - Silver nanoparticles
KW - Silver nitrate
KW - Two-dimensional electrophoresis
UR - http://www.scopus.com/inward/record.url?scp=85066858504&partnerID=8YFLogxK
U2 - 10.1007/s11356-019-05552-w
DO - 10.1007/s11356-019-05552-w
M3 - Article
C2 - 31161543
AN - SCOPUS:85066858504
SN - 0944-1344
VL - 26
SP - 22529
EP - 22550
JO - Environmental Science and Pollution Research International
JF - Environmental Science and Pollution Research International
IS - 22
ER -