Compact and low-cost fluorescence based flow-through analyzer for early-stage classification of potentially toxic algae and in situ semi-quantification

Silvia Zieger, Günter Mistlberger, Lukas Troi, Alexander Lang, Fabio Confalonieri, Ingo Klimant

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

The occurrence and intensity of (harmful) algal blooms (HABs) have increased through the years due to rapidly changing environmental conditions. At the same time, the demand for low-cost instrumen-tation has increased substantially, enabling real-time monitoring and early stage detection of HABs. To meet this challenge, we have developed a compact multi-wavelength fluorometer for less than 400 USD. This is possible by using readily available, low-cost optical and electronic components. Its modular design results in a highly versatile and flexible monitoring tool. The algae detection module enables a continuous identification and control of relevant algal groups based on their spectral charac-teristics with a detection limit of 10 cells/L. Besides its usage as a benchtop module in the laboratory, the algae module has been integrated into submersible housings and applied in coastal environments. During its first in situ application in the Port of Genoa, seawater samples of mixed algal composition were used to demonstrate the successful discrimination of cyanobacteria and dinophytes as well-known toxin producing classes. The fabrica-tion, operation and performance as well as its first in situ application are addressed.

Originalspracheenglisch
FachzeitschriftEnvironmental science & technology
DOIs
PublikationsstatusElektronische Veröffentlichung vor Drucklegung. - 3 Jun 2018

Fingerprint

Poisons
Algae
fluorescence
Fluorescence
alga
Fluorometers
Monitoring
algal bloom
Seawater
cost
Costs
submersible
monitoring
Wavelength
toxin
coastal zone
cyanobacterium
Chemical analysis
environmental conditions
seawater

Schlagwörter

    Dies zitieren

    Compact and low-cost fluorescence based flow-through analyzer for early-stage classification of potentially toxic algae and in situ semi-quantification. / Zieger, Silvia; Mistlberger, Günter; Troi, Lukas; Lang, Alexander; Confalonieri, Fabio; Klimant, Ingo.

    in: Environmental science & technology, 03.06.2018.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    @article{5fc7336ceae4440dbc3810a924b38668,
    title = "Compact and low-cost fluorescence based flow-through analyzer for early-stage classification of potentially toxic algae and in situ semi-quantification",
    abstract = "The occurrence and intensity of (harmful) algal blooms (HABs) have increased through the years due to rapidly changing environmental conditions. At the same time, the demand for low-cost instrumen-tation has increased substantially, enabling real-time monitoring and early stage detection of HABs. To meet this challenge, we have developed a compact multi-wavelength fluorometer for less than 400 USD. This is possible by using readily available, low-cost optical and electronic components. Its modular design results in a highly versatile and flexible monitoring tool. The algae detection module enables a continuous identification and control of relevant algal groups based on their spectral charac-teristics with a detection limit of 10 cells/L. Besides its usage as a benchtop module in the laboratory, the algae module has been integrated into submersible housings and applied in coastal environments. During its first in situ application in the Port of Genoa, seawater samples of mixed algal composition were used to demonstrate the successful discrimination of cyanobacteria and dinophytes as well-known toxin producing classes. The fabrica-tion, operation and performance as well as its first in situ application are addressed.",
    keywords = "Journal Article",
    author = "Silvia Zieger and G{\"u}nter Mistlberger and Lukas Troi and Alexander Lang and Fabio Confalonieri and Ingo Klimant",
    year = "2018",
    month = "6",
    day = "3",
    doi = "10.1021/acs.est.8b00578",
    language = "English",
    journal = "Environmental science & technology",
    issn = "0013-936X",
    publisher = "American Chemical Society",

    }

    TY - JOUR

    T1 - Compact and low-cost fluorescence based flow-through analyzer for early-stage classification of potentially toxic algae and in situ semi-quantification

    AU - Zieger, Silvia

    AU - Mistlberger, Günter

    AU - Troi, Lukas

    AU - Lang, Alexander

    AU - Confalonieri, Fabio

    AU - Klimant, Ingo

    PY - 2018/6/3

    Y1 - 2018/6/3

    N2 - The occurrence and intensity of (harmful) algal blooms (HABs) have increased through the years due to rapidly changing environmental conditions. At the same time, the demand for low-cost instrumen-tation has increased substantially, enabling real-time monitoring and early stage detection of HABs. To meet this challenge, we have developed a compact multi-wavelength fluorometer for less than 400 USD. This is possible by using readily available, low-cost optical and electronic components. Its modular design results in a highly versatile and flexible monitoring tool. The algae detection module enables a continuous identification and control of relevant algal groups based on their spectral charac-teristics with a detection limit of 10 cells/L. Besides its usage as a benchtop module in the laboratory, the algae module has been integrated into submersible housings and applied in coastal environments. During its first in situ application in the Port of Genoa, seawater samples of mixed algal composition were used to demonstrate the successful discrimination of cyanobacteria and dinophytes as well-known toxin producing classes. The fabrica-tion, operation and performance as well as its first in situ application are addressed.

    AB - The occurrence and intensity of (harmful) algal blooms (HABs) have increased through the years due to rapidly changing environmental conditions. At the same time, the demand for low-cost instrumen-tation has increased substantially, enabling real-time monitoring and early stage detection of HABs. To meet this challenge, we have developed a compact multi-wavelength fluorometer for less than 400 USD. This is possible by using readily available, low-cost optical and electronic components. Its modular design results in a highly versatile and flexible monitoring tool. The algae detection module enables a continuous identification and control of relevant algal groups based on their spectral charac-teristics with a detection limit of 10 cells/L. Besides its usage as a benchtop module in the laboratory, the algae module has been integrated into submersible housings and applied in coastal environments. During its first in situ application in the Port of Genoa, seawater samples of mixed algal composition were used to demonstrate the successful discrimination of cyanobacteria and dinophytes as well-known toxin producing classes. The fabrica-tion, operation and performance as well as its first in situ application are addressed.

    KW - Journal Article

    U2 - 10.1021/acs.est.8b00578

    DO - 10.1021/acs.est.8b00578

    M3 - Article

    JO - Environmental science & technology

    JF - Environmental science & technology

    SN - 0013-936X

    ER -