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Abstract
The serum amyloid A (SAA) family of proteins is encoded by multiple genes, which display allelic variation and a high degree of homology in mammals. The SAA1/2 genes code for non-glycosylated acute-phase SAA1/2 proteins, that may increase up to 1000-fold during inflammation. The SAA4 gene, well characterized in humans (hSAA4) and mice (mSaa4) codes for a SAA4 protein that is glycosylated only in humans. We here report on a previously uncharacterized SAA4 gene (rSAA4) and its product in Rattus norvegicus, the only mammalian species known not to express acute-phase SAA. The exon/intron organization of rSAA4 is similar to that reported for hSAA4 and mSaa4. By performing 5′- and 3′RACE, we identified a 1830-bases containing rSAA4 mRNA (including a GA-dinucleotide tandem repeat). Highest rSAA4 mRNA expression was detected in rat liver. In McA-RH7777 rat hepatoma cells, rSAA4 transcription was significantly upregulated in response to LPS and IL-6 while IL-1α/β and TNFα were without effect. Luciferase assays with promoter-truncation constructs identified three proximal C/EBP-elements that mediate expression of rSAA4 in McA-RH7777 cells. In line with sequence prediction a 14-kDa non-glycosylated SAA4 protein is abundantly expressed in rat liver. Fluorescence microscopy revealed predominant localization of rSAA4-GFP-tagged fusion protein in the ER.
Originalsprache | englisch |
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Seiten (von - bis) | 1643-1649 |
Seitenumfang | 7 |
Fachzeitschrift | Biochemical and Biophysical Research Communications |
Jahrgang | 450 |
Ausgabenummer | 4 |
DOIs | |
Publikationsstatus | Veröffentlicht - 8 Aug. 2014 |
ASJC Scopus subject areas
- Biophysik
- Biochemie
- Molekularbiologie
- Zellbiologie
Fields of Expertise
- Human- & Biotechnology
Treatment code (Nähere Zuordnung)
- Basic - Fundamental (Grundlagenforschung)
- Experimental
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FWF - APMAP in adipogenesis - Die Rolle von APMAP in der Adipogenese und im Energiehaushal
Huber, K., Pessentheiner, A., Prokesch, A. & Bogner-Strauß, J. G.
1/03/12 → 31/12/15
Projekt: Forschungsprojekt