Characterisation of Candida within the Mycobiome/Microbiome of the Lower Respiratory Tract of ICU Patients

Robert Krause, Bettina Halwachs, Gerhard Thallinger, Ingeborg Klymiuk, Gregor Gorkiewicz, Martin Hoenigl, Jürgen Prattes, Thomas Valentin, Katharina Heidrich, Walter Buzina, Helmut J F Salzer, Jasmin Rabensteiner, Florian Prüller, Reinhard B Raggam, Andreas Meinitzer, Christine Moissl-Eichinger, Christoph Högenauer, Franz Quehenberger, Karl Kashofer, Ines Zollner-Schwetz

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Whether the presence of Candida spp. in lower respiratory tract (LRT) secretions is a marker of underlying disease, intensive care unit (ICU) treatment and antibiotic therapy or contributes to poor clinical outcome is unclear. We investigated healthy controls, patients with proposed risk factors for Candida growth in LRT (antibiotic therapy, ICU treatment with and without antibiotic therapy), ICU patients with pneumonia and antibiotic therapy and candidemic patients (for comparison of truly invasive and colonizing Candida spp.). Fungal patterns were determined by conventional culture based microbiology combined with molecular approaches (next generation sequencing, multilocus sequence typing) for description of fungal and concommitant bacterial microbiota in LRT, and host and fungal biomarkes were investigated. Admission to and treatment on ICUs shifted LRT fungal microbiota to Candida spp. dominated fungal profiles but antibiotic therapy did not. Compared to controls, Candida was part of fungal microbiota in LRT of ICU patients without pneumonia with and without antibiotic therapy (63% and 50% of total fungal genera) and of ICU patients with pneumonia with antibiotic therapy (73%) (p<0.05). No case of invasive candidiasis originating from Candida in the LRT was detected. There was no common bacterial microbiota profile associated or dissociated with Candida spp. in LRT. Colonizing and invasive Candida strains (from candidemic patients) did not match to certain clades withdrawing the presence of a particular pathogenic and invasive clade. The presence of Candida spp. in the LRT rather reflected rapidly occurring LRT dysbiosis driven by ICU related factors than was associated with invasive candidiasis.

Originalspracheenglisch
Aufsatznummere0155033
Seiten (von - bis)e0155033
FachzeitschriftPLoS ONE
Jahrgang11
Ausgabenummer5
DOIs
PublikationsstatusVeröffentlicht - 20 Mai 2016

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Intensive care units
Candida
Microbiota
respiratory system
Respiratory System
Intensive Care Units
antibiotics
Anti-Bacterial Agents
therapeutics
pneumonia
Invasive Candidiasis
candidiasis
Therapeutics
Pneumonia
Dysbiosis
Mycobiome
microbiome
Microbiology
Multilocus Sequence Typing
microbiology

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    Krause, R., Halwachs, B., Thallinger, G., Klymiuk, I., Gorkiewicz, G., Hoenigl, M., ... Zollner-Schwetz, I. (2016). Characterisation of Candida within the Mycobiome/Microbiome of the Lower Respiratory Tract of ICU Patients. PLoS ONE, 11(5), e0155033. [e0155033]. https://doi.org/10.1371/journal.pone.0155033

    Characterisation of Candida within the Mycobiome/Microbiome of the Lower Respiratory Tract of ICU Patients. / Krause, Robert; Halwachs, Bettina; Thallinger, Gerhard; Klymiuk, Ingeborg; Gorkiewicz, Gregor; Hoenigl, Martin; Prattes, Jürgen; Valentin, Thomas; Heidrich, Katharina; Buzina, Walter; Salzer, Helmut J F; Rabensteiner, Jasmin; Prüller, Florian; Raggam, Reinhard B; Meinitzer, Andreas; Moissl-Eichinger, Christine; Högenauer, Christoph; Quehenberger, Franz; Kashofer, Karl; Zollner-Schwetz, Ines.

    in: PLoS ONE, Jahrgang 11, Nr. 5, e0155033, 20.05.2016, S. e0155033.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Krause, R, Halwachs, B, Thallinger, G, Klymiuk, I, Gorkiewicz, G, Hoenigl, M, Prattes, J, Valentin, T, Heidrich, K, Buzina, W, Salzer, HJF, Rabensteiner, J, Prüller, F, Raggam, RB, Meinitzer, A, Moissl-Eichinger, C, Högenauer, C, Quehenberger, F, Kashofer, K & Zollner-Schwetz, I 2016, 'Characterisation of Candida within the Mycobiome/Microbiome of the Lower Respiratory Tract of ICU Patients' PLoS ONE, Jg. 11, Nr. 5, e0155033, S. e0155033. https://doi.org/10.1371/journal.pone.0155033
    Krause, Robert ; Halwachs, Bettina ; Thallinger, Gerhard ; Klymiuk, Ingeborg ; Gorkiewicz, Gregor ; Hoenigl, Martin ; Prattes, Jürgen ; Valentin, Thomas ; Heidrich, Katharina ; Buzina, Walter ; Salzer, Helmut J F ; Rabensteiner, Jasmin ; Prüller, Florian ; Raggam, Reinhard B ; Meinitzer, Andreas ; Moissl-Eichinger, Christine ; Högenauer, Christoph ; Quehenberger, Franz ; Kashofer, Karl ; Zollner-Schwetz, Ines. / Characterisation of Candida within the Mycobiome/Microbiome of the Lower Respiratory Tract of ICU Patients. in: PLoS ONE. 2016 ; Jahrgang 11, Nr. 5. S. e0155033.
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    abstract = "Whether the presence of Candida spp. in lower respiratory tract (LRT) secretions is a marker of underlying disease, intensive care unit (ICU) treatment and antibiotic therapy or contributes to poor clinical outcome is unclear. We investigated healthy controls, patients with proposed risk factors for Candida growth in LRT (antibiotic therapy, ICU treatment with and without antibiotic therapy), ICU patients with pneumonia and antibiotic therapy and candidemic patients (for comparison of truly invasive and colonizing Candida spp.). Fungal patterns were determined by conventional culture based microbiology combined with molecular approaches (next generation sequencing, multilocus sequence typing) for description of fungal and concommitant bacterial microbiota in LRT, and host and fungal biomarkes were investigated. Admission to and treatment on ICUs shifted LRT fungal microbiota to Candida spp. dominated fungal profiles but antibiotic therapy did not. Compared to controls, Candida was part of fungal microbiota in LRT of ICU patients without pneumonia with and without antibiotic therapy (63{\%} and 50{\%} of total fungal genera) and of ICU patients with pneumonia with antibiotic therapy (73{\%}) (p<0.05). No case of invasive candidiasis originating from Candida in the LRT was detected. There was no common bacterial microbiota profile associated or dissociated with Candida spp. in LRT. Colonizing and invasive Candida strains (from candidemic patients) did not match to certain clades withdrawing the presence of a particular pathogenic and invasive clade. The presence of Candida spp. in the LRT rather reflected rapidly occurring LRT dysbiosis driven by ICU related factors than was associated with invasive candidiasis.",
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    AU - Gorkiewicz, Gregor

    AU - Hoenigl, Martin

    AU - Prattes, Jürgen

    AU - Valentin, Thomas

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    AU - Buzina, Walter

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    AU - Meinitzer, Andreas

    AU - Moissl-Eichinger, Christine

    AU - Högenauer, Christoph

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