Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules

Kang Liang, Raffaele Ricco, Cara M. Doherty, Mark J. Styles, Stephen Bell, Nigel Kirby, Stephen Mudie, David Haylock, Anita J. Hill, Christian J. Doonan, Paolo Falcaro

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Enhancing the robustness of functional biomacromolecules is a critical challenge in biotechnology, which if addressed would enhance their use in pharmaceuticals, chemical processing and biostorage. Here we report a novel method, inspired by natural biomineralization processes, which provides unprecedented protection of biomacromolecules by encapsulating them within a class of porous materials termed metal-organic frameworks. We show that proteins, enzymes and DNA rapidly induce the formation of protective metal-organic framework coatings under physiological conditions by concentrating the framework building blocks and facilitating crystallization around the biomacromolecules. The resulting biocomposite is stable under conditions that would normally decompose many biological macromolecules. For example, urease and horseradish peroxidase protected within a metal-organic framework shell are found to retain bioactivity after being treated at 80°C and boiled in dimethylformamide (153°C), respectively. This rapid, low-cost biomimetic mineralization process gives rise to new possibilities for the exploitation of biomacromolecules.

Originalspracheenglisch
Aufsatznummer7240
FachzeitschriftNature Communications
Jahrgang6
DOIs
PublikationsstatusVeröffentlicht - 4 Jun 2015
Extern publiziertJa

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Biomimetics
protective coatings
biomimetics
Protective coatings
Metals
Biomineralization
Metal coatings
Dimethylformamide
Organic coatings
Urease
Horseradish Peroxidase
Biotechnology
Crystallization
Bioactivity
Macromolecules
metals
Porous materials
biotechnology
encapsulating
concentrating

ASJC Scopus subject areas

  • !!Biochemistry, Genetics and Molecular Biology(all)
  • !!Chemistry(all)
  • !!Physics and Astronomy(all)

Dies zitieren

Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules. / Liang, Kang; Ricco, Raffaele; Doherty, Cara M.; Styles, Mark J.; Bell, Stephen; Kirby, Nigel; Mudie, Stephen; Haylock, David; Hill, Anita J.; Doonan, Christian J.; Falcaro, Paolo.

in: Nature Communications , Jahrgang 6, 7240, 04.06.2015.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Liang, K, Ricco, R, Doherty, CM, Styles, MJ, Bell, S, Kirby, N, Mudie, S, Haylock, D, Hill, AJ, Doonan, CJ & Falcaro, P 2015, 'Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules' Nature Communications , Jg. 6, 7240. https://doi.org/10.1038/ncomms8240
Liang, Kang ; Ricco, Raffaele ; Doherty, Cara M. ; Styles, Mark J. ; Bell, Stephen ; Kirby, Nigel ; Mudie, Stephen ; Haylock, David ; Hill, Anita J. ; Doonan, Christian J. ; Falcaro, Paolo. / Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules. in: Nature Communications . 2015 ; Jahrgang 6.
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