Activity‐Based Protein Profiling (ABPP) of Oxidoreductases

Rita Fürst, Rolf Breinbauer*

*Korrespondierende/r Autor/in für diese Arbeit

Publikation: Beitrag in einer FachzeitschriftReview eines Fachbereichs (Review article)Begutachtung

Abstract

Over the last two decades, activity-based protein profiling (ABPP) has been established as a tremendously useful proteomic tool for measuring the activity of proteins in their cellular context, annotating the function of uncharacterized proteins, and investigating the target profile of small-molecule inhibitors. Unlike hydrolases and other enzyme classes, which exhibit a characteristic nucleophilic residue, oxidoreductases have received much less attention in ABPP. In this minireview, the state of the art of ABPP of oxidoreductases is described and the scope and limitations of the existing approaches are discussed. It is noted that several ABPP probes have been described for various oxidases, but none so far for a reductase, which gives rise to opportunities for future research.

Originalspracheenglisch
Seiten (von - bis)630–638
Seitenumfang9
FachzeitschriftChemBioChem
Jahrgang22
Ausgabenummer4
Frühes Online-Datum2 Sep 2020
DOIs
PublikationsstatusVeröffentlicht - 15 Feb 2021

ASJC Scopus subject areas

  • Molekularmedizin
  • Molekularbiologie
  • Biochemie
  • Organische Chemie

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