A mechanistic explanation of the inhibitory role of the protein corona on liposomal gene expression

Sara Palchetti, Luca Digiacomo, Francesca Giulimondi, Daniela Pozzi, Giovanna Peruzzi, Gianmarco Ferri, Heinz Amenitsch, Francesco Cardarelli, Morteza Mahmoudi, Giulio Caracciolo

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

The past three decades have witnessed fast advances in the use of cationic liposome-DNA complexes (lipoplexes) for gene delivery applications. However, no lipoplex formulation has reached into the clinical practice so far. The primary drawback limiting clinical use of lipoplexes is the lack of mechanistic understanding of their low transfection efficiency (TE) in vivo. In physiological environments, lipoplexes are coated by a protein corona (PC) that mediates the interactions with the cell machinery. Here we show that the formation of PC can change the interactions of multicomponent (MC) lipoplexes with our cell model (i.e., HeLa). At the highest lipoplex concentration, the formation of PC can reduce the TE of MC lipoplexes from 60% to <5%. Combining dynamic light scattering and synchrotron small-angle X-ray scattering (SAXS), we clarify that the formation of PC modifies physical-chemical properties of MC lipoplexes so as to affect their TE. Moreover, we examined single transfection barriers by a combination of fluorescence-activated cell sorting, single-cell real-time fluorescence confocal microscopy, and synchrotron SAXS. We demonstrate that PC formation has the ability to modify the relative contribution of caveolae-mediated endocytosis and macropinocytosis in lipoplexes uptake, in favor of the latter, increasing accumulation of PC-decorated lipoplexes into degradative lysosomal compartments. Finally, we report evidences that PC reduces the structural stability of lipoplexes against solubilization by cellular lipids, likely favoring premature DNA release and cytosolic digestion by DNAase. These combined effects revealed here offer a comprehensive mechanistic explanation on the reason behind reduction in gene expression of MC lipoplexes.

Originalspracheenglisch
Aufsatznummer183159
FachzeitschriftBiochimica et Biophysica Acta - Biomembranes
Jahrgang1862
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - 1 Mär 2020

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Gene expression
Liposomes
Gene Expression
DNA
Transfection
Synchrotrons
X ray scattering
Protein Corona
X-Rays
Caveolae
Deoxyribonucleases
Confocal microscopy
Fluorescence microscopy
Dynamic light scattering
Endocytosis
Fluorescence Microscopy
Sorting
Confocal Microscopy
Cell Communication
Chemical properties

Schlagwörter

    ASJC Scopus subject areas

    • !!Biophysics
    • !!Biochemistry
    • Zellbiologie

    Dies zitieren

    Palchetti, S., Digiacomo, L., Giulimondi, F., Pozzi, D., Peruzzi, G., Ferri, G., ... Caracciolo, G. (2020). A mechanistic explanation of the inhibitory role of the protein corona on liposomal gene expression. Biochimica et Biophysica Acta - Biomembranes, 1862(3), [183159]. https://doi.org/10.1016/j.bbamem.2019.183159

    A mechanistic explanation of the inhibitory role of the protein corona on liposomal gene expression. / Palchetti, Sara; Digiacomo, Luca; Giulimondi, Francesca; Pozzi, Daniela; Peruzzi, Giovanna; Ferri, Gianmarco; Amenitsch, Heinz; Cardarelli, Francesco; Mahmoudi, Morteza; Caracciolo, Giulio.

    in: Biochimica et Biophysica Acta - Biomembranes, Jahrgang 1862, Nr. 3, 183159, 01.03.2020.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Palchetti, S, Digiacomo, L, Giulimondi, F, Pozzi, D, Peruzzi, G, Ferri, G, Amenitsch, H, Cardarelli, F, Mahmoudi, M & Caracciolo, G 2020, 'A mechanistic explanation of the inhibitory role of the protein corona on liposomal gene expression' Biochimica et Biophysica Acta - Biomembranes, Jg. 1862, Nr. 3, 183159. https://doi.org/10.1016/j.bbamem.2019.183159
    Palchetti, Sara ; Digiacomo, Luca ; Giulimondi, Francesca ; Pozzi, Daniela ; Peruzzi, Giovanna ; Ferri, Gianmarco ; Amenitsch, Heinz ; Cardarelli, Francesco ; Mahmoudi, Morteza ; Caracciolo, Giulio. / A mechanistic explanation of the inhibitory role of the protein corona on liposomal gene expression. in: Biochimica et Biophysica Acta - Biomembranes. 2020 ; Jahrgang 1862, Nr. 3.
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    abstract = "The past three decades have witnessed fast advances in the use of cationic liposome-DNA complexes (lipoplexes) for gene delivery applications. However, no lipoplex formulation has reached into the clinical practice so far. The primary drawback limiting clinical use of lipoplexes is the lack of mechanistic understanding of their low transfection efficiency (TE) in vivo. In physiological environments, lipoplexes are coated by a protein corona (PC) that mediates the interactions with the cell machinery. Here we show that the formation of PC can change the interactions of multicomponent (MC) lipoplexes with our cell model (i.e., HeLa). At the highest lipoplex concentration, the formation of PC can reduce the TE of MC lipoplexes from 60{\%} to <5{\%}. Combining dynamic light scattering and synchrotron small-angle X-ray scattering (SAXS), we clarify that the formation of PC modifies physical-chemical properties of MC lipoplexes so as to affect their TE. Moreover, we examined single transfection barriers by a combination of fluorescence-activated cell sorting, single-cell real-time fluorescence confocal microscopy, and synchrotron SAXS. We demonstrate that PC formation has the ability to modify the relative contribution of caveolae-mediated endocytosis and macropinocytosis in lipoplexes uptake, in favor of the latter, increasing accumulation of PC-decorated lipoplexes into degradative lysosomal compartments. Finally, we report evidences that PC reduces the structural stability of lipoplexes against solubilization by cellular lipids, likely favoring premature DNA release and cytosolic digestion by DNAase. These combined effects revealed here offer a comprehensive mechanistic explanation on the reason behind reduction in gene expression of MC lipoplexes.",
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    AU - Giulimondi, Francesca

    AU - Pozzi, Daniela

    AU - Peruzzi, Giovanna

    AU - Ferri, Gianmarco

    AU - Amenitsch, Heinz

    AU - Cardarelli, Francesco

    AU - Mahmoudi, Morteza

    AU - Caracciolo, Giulio

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