Veröffentlichungen suchen

Suchergebnisse

• 2020

  On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

  Bolivar, J. M. & Nidetzky, B., 1 Feb 2020, in : Biochimica et Biophysica Acta - Proteins and Proteomics. 1868, 2, 140333.

  Publikation: Beitrag in einer Fachzeitschrift › Review eines Fachbereichs (Review article)

  • Immobilized Enzymes
  • Molecules
  • Enzymes
  • Immobilization
  • Catalytic Domain

• Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

  Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, in : Methods in molecular biology (Clifton, N.J.). 2100, S. 319-333 15 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Immobilized Enzymes
  • Proteins
  • Enzymes
  • Penicillin Amidase
  • Proton-Motive Force
• 2012

  Non-native aggregation of recombinant human granulocyte-colony stimulating factor under simulated process stress conditions

  Rößl, U., Wiesbauer, J., Leitgeb, S., Birner-Grünberger, R. & Nidetzky, B., 2012, in : Biotechnology Journal. 7, 8, S. 1014-1024

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2015

  Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

  Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, in : Chemical Communications. S. 3083-3086

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2013

  Development chain of bioreductions: Catalyst design, reaction engineering, operation mode, decision guidance

  Kratzer, R., Binder, W. & Nidetzky, B., 13 Okt 2013.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2012

  Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

  Kratzer, R., Mädje, K. T. C. & Nidetzky, B., 6 Nov 2012.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2013

  Exploring the Active Binding Site of Nitrile Reductases

  Wilding, B., Petschacher, B., Winkler, M., Offenmüller, G., Kratzer, R., Steinkellner, G., Lyskowski, A. F., Gruber, K., Nidetzky, B. & Klempier, N., 10 Sep 2013.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation

• From NMR to enzymatic information

  Vogl, M., Kratzer, R., Nidetzky, B. & Brecker, L., 21 Apr 2013.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2017

  Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, in : Angewandte Chemie. 129, S. 2544 –2548

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2014

  Acceleration of an aldo-keto reductase by minimal loop engineering

  Krump, C., Vogl, M., Brecker, L., Nidetzky, B. & Kratzer, R., 2014, in : Protein engineering, design and selection. 27, S. 245-248

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2015

  Rules for biocatalyst and reaction engineering to implement effective, NAD(P)H-dependent, whole cell bioreductions

  Kratzer, R., Woodley, J. & Nidetzky, B., 2015, in : Biotechnology Advances. 33, 8, S. 1641–1652

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2013

  Biological nitrile reduction – exploring a remarkable reaction

  Wilding, B., Petschacher, B., Winkler, M., Kratzer, R., Steinkellner, G., Lyskowski, A. F., Gruber, K., Nidetzky, B. & Klempier, N., 23 Sep 2013.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2012

  NMR studies of ternary carbohydrate - NAD(H)- Candida tenuis xylose reductase complexes

  Vogl, M., Kratzer, R., Nidetzky, B. & Brecker, L., 22 Apr 2012.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation

• Host cell and expression engineering for development of an E. coli ketoreductase catalyst: Enhancement of formate dehydrogenase activity for regeneration of NADH

  Mädje, K. T., Schmoelzer, K., Nidetzky, B. & Kratzer, R., 2012, in : Microbial cell factories . 11, S. 1-7

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2013

  Freezing Process Development under QbD Principles - In-silico Solutions for Process Characterization and Scaling

  Rößl, U., Leitgeb, S., Jajcevic, D. & Nidetzky, B., 2013.

  Publikation: Konferenzbeitrag › Poster
• 2014

  Dissecting Physical and Biochemical Factors of Catalytic Effectiveness in Immobilized D-Amino Acid Oxidase by Real-Time Sensing of O2 Availability Inside Porous Carriers

  Bolivar Bolivar, J. M., Schelch, S., Mayr, T. & Nidetzky, B., 2014, in : ChemCatChem. 6, 4, S. 981-986

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • D-Amino-Acid Oxidase
  • physical factors
  • oxidase
  • availability
  • amino acids
• 2016

  β-Cyclodextrin Improves Solubility and Enzymatic C-Glucosylation of the Flavonoid Phloretin

  Bungaruang, L., Gutmann, A. & Nidetzky, B., 2016, in : Advanced Synthesis & Catalysis. 358, 3, S. 486-493

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Phloretin
  • Flavonoids
  • Cyclodextrins
  • Solubility
  • Complexation
• 2013

  Smart enzyme immobilization in microstructured reactors

  Bolivar, J. M. & Nidetzky, B., Mai 2013, in : Chimica Oggi. 31, 3, S. 50-54 5 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Enzyme immobilization
  • Immobilized Enzymes
  • Enzymes
  • Microstructure
  • Biotransformation
• 2017

  Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

  Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, in : Biotechnology Journal.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Uridine Diphosphate Glucose
  • Glycosyltransferases
  • Uridine Diphosphate
  • Recycling
  • Biological Products
• 2013

  Shine a light on immobilized enzymes: Real-time sensing in solid supported biocatalysts

  Bolivar, J. M., Consolati, T., Mayr, T. & Nidetzky, B., Mär 2013, in : Trends in Biotechnology. 31, 3, S. 194-203 10 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Immobilized Enzymes
  • Biocatalysts
  • Enzymes
  • Immobilization
  • Catalyst supports
• 2012

  Structural and kinetic evidence that the catalytic reaction of human UDP-glucose 6-dehydrogenase involves covalent thiohemiacetal and thioester enzyme intermediates

  Egger, S., Chaikuad, A., Klimacek, M., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 2012, in : The Journal of Biological Chemistry. 287, S. 2119-2129

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2014

  Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

  Klimacek, M., Kirl, E., Krahulec, S., Longus, K., Novy, V. & Nidetzky, B., 2014, in : Microbial cell factories . 13, 37, S. 1-12

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2012

  Structure and mechanism of human UDP-xylose synthase: evidence for a promoting role of sugar ring distortion in a three-step catalytic conversion of UDP-glucuronic acid

  Eixelsberger, T., Sykora, S., Egger, S., Brunsteiner, M., Kavanagh, K., Oppermann, U., Brecker, L. & Nidetzky, B., 2012, in : The Journal of Biological Chemistry. 287, 37, S. 31349-31358

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2019

  Sialic acid aldolase and synthase compared in optimized multi-enzyme cascade production of 3'-sialyllactose

  Schelch, S., Gross Belduma, S. M., Eibinger, M., Kuballa, J., Petschacher, B. & Nidetzky, B., 18 Nov 2019.

  Publikation: Konferenzbeitrag › Poster
• 2012

  Structure and mechanism of human UDP-xylose synthase

  Eixelsberger, T., Sykora, S., Egger, S., Brunsteiner, M., Kavanagh, K., Oppermann, U., Brecker, L. & Nidetzky, B., 21 Mai 2012.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2017

  Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

  Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, in : Angewandte Chemie / International Edition . 56, S. 2503 –2507

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Xylose
  • Uridine Diphosphate
  • Isotopes
  • Enzymes
  • Sugars
• 2020

  On the donor substrate dependence of group‐transfer reactions by hydrolytic enzymes: Insight from kinetic analysis of sucrose phosphorylase‐catalyzed transglycosylation

  Klimacek, M., Sigg, A. & Nidetzky, B., 23 Jun 2020, in : Biotechnology and Bioengineering. 2020, 117

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

• A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 27 Mai 2020, in : ACS Central Science. 6, 5, S. 739-746 8 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  Open Access

  • Cellulose
  • Crystalline materials
  • Nanocrystals
  • Cellulases
  • Degradation
• 2017

  Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

  Gudiminchi, R. K. & Nidetzky, B., 18 Jul 2017, in : ChemBioChem. 18, 14, S. 1387-1390 4 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • sucrose phosphorylase
  • Ascorbic Acid
  • Sucrose
  • Cosmetics
  • Protonation
• 2012

  Dual-lifetime referencing (DLR): a powerful method for on-line measurement of internal pH in carrier-bound immobilized biocatalysts

  Boniello, C., Mayr, T., Bolivar Bolivar, J. M. & Nidetzky, B., 2012, in : BMC Biotechnology . 12, 11, S. 1-10

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2014

  Tunable close-to-nature cellulose for enzymatic degradation studies via dynamic high resolution AFM

  Ganner, T., Rosker, S., Eibinger, M., Chernev, B. S., Kraxner, J., Mayrhofer, C., Aschl, T., Zahel, T., Bubner, P., Nidetzky, B. & Plank, H., 7 Sep 2014.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2015

  Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

  Wildberger, P., Pfeiffer, M., Brecker, L. & Nidetzky, B., 21 Dez 2015, in : Angewandte Chemie / International Edition . 54, 52, S. 15867-15871 5 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Phosphorylase Phosphatase
  • Phosphatases
  • Phosphates
  • Phosphorylation
  • Catalysts
• 2016

  Unlocking the Potential of Leloir Glycosyltransferases for Applied Biocatalysis: Efficient Synthesis of Uridine 5′-Diphosphate-Glucose by Sucrose Synthase

  Gutmann, A. & Nidetzky, B., 25 Okt 2016, in : Advanced Synthesis & Catalysis. 358, S. 3600-3609

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Glycosyltransferases
  • Diphosphates
  • Uridine
  • Sugar (sucrose)
  • Glucose
• 2015

  Tunable Semicrystalline Thin Film Cellulose Substrate for High- Resolution, In-Situ AFM Characterization of Enzymatic Cellulose Degradation

  Ganner, T., Rosker, S., Eibinger, M., Kraxner, J., Sattelkow, J., Rattenberger, J., Fitzek, H. M., Chernev, B. S., Grogger, W., Nidetzky, B. & Plank, H., 2015, in : ACS Applied Materials & Interfaces. 7, 50, S. 27900-27909

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2012

  Enzymatic production of 2-O-(alpha-D-glucopyranosyl)-glycerate using sucrose phosphorylase

  Goedl, C., Sawangwan, T. & Nidetzky, B., 18 Jul 2012, Patent Nr. EP 2186904

  Publikation: Schutzrecht › Patent

• A novel multifunctional sialyltransferase PdST from Pasteurella dagmatis

  Schmoelzer, K., Kokot, D., Czabany, T., Luley, C., Ribitsch, D., Zitzenbacher, S., Augustin, P. A., Lyskowski, A. F., Nidetzky, B. & Schwab, H., 2 Jul 2012.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2013

  A newly discovered multifunctional alpha2,3-sialyltransferase from Pasteurella dagmatis carries a natural Ser-to-Thr substitution

  Schmoelzer, K., Ribitsch, D., Czabany, T., Luley, C., Kokot, D., Lyskowski, A. F., Zitzenbacher, S., Schwab, H. & Nidetzky, B., 7 Jul 2013.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation

  Open Access

  Datei
• 2019

  Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

  Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, in : Organic and Biomolecular Chemistry. 17, 23, S. 5661-5665 5 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  Open Access

  • Hexosaminidases
  • Human Milk
  • Lactose
  • Oligosaccharides
  • Glycosylation

• Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

  Zhong, C., Luley-Goedl, C. & Nidetzky, B., 1 Jan 2019, in : Biotechnology and Bioengineering. 116, 9, S. 2146-2155 10 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  Open Access

  • cellodextrin phosphorylase
  • cellobiose phosphorylase
  • Polymerization
  • Solubility
  • Glucose
• 2013

  Quantitating intraparticle O2 gradients in solid supported enzyme immobilizates: experimental determination of their role in limiting the catalytic effectiveness of immobilized glucose oxidase.

  Bolivar Bolivar, J. M., Consolati, T., Mayr, T. & Nidetzky, B., 2013, in : Biotechnology and Bioengineering. 110, 8, S. 2086-2095

  Publikation: Beitrag in einer Fachzeitschrift › Artikel
• 2015

  Action of an AA9 - type lytic polysaccharide monooxygenase on the cellulose surface and its effect on cellulase hydrolytic efficiency

  Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 2 Aug 2015.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2017

  Debottlenecking enzymatic production of fucosylated HMOs with prokaryotic a1,2-fucosyltransferases

  Petschacher, B., Gierlinger, K., Brunner, L., Kuballa, J. & Nidetzky, B., 23 Apr 2017.

  Publikation: Konferenzbeitrag › Poster
• 2014

  Prebiotic

  Nidetzky, B. & Schwarz, A., 11 Jun 2014, Patent Nr. EP 2509450 A1

  Publikation: Schutzrecht › Patent
• 2012

  Characterization and biosynthetic application of human UDP-xylose synthase

  Eixelsberger, T. & Nidetzky, B., 10 Dez 2012.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2016

  Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

  Lemmerer, M., Schmoelzer, K., Gutmann, A. & Nidetzky, B., 2016, in : Advanced Synthesis & Catalysis. 358, S. 3113-3122

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  • Sugar (sucrose)
  • Nucleosides
  • Sugars
  • Chromatography
  • Anions
• 2019

  Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

  Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, in : Catalysis Science & Technology. 9, 3, S. 842-853 12 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  Open Access

  • Nitriles
  • Proton transfer
  • Catalysis
  • Escherichia coli
  • Protons
• 2012

  Cellulases dig deep: in situ observation of the mesoscopic structural dynamics of enzymatic cellulose degradation

  Bubner, P., Ganner, T., Eibinger, M., Dohr, J., Mayrhofer, C., Plank, H. & Nidetzky, B., 10 Okt 2012.

  Publikation: Konferenzbeitrag › (Altdaten) Vortrag oder Präsentation
• 2019

  A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

  Pfeiffer, M., Johansson, C., Krojer, T., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 5 Apr 2019, in : ACS Catalysis. 9, 4, S. 2962-2968 7 S.

  Publikation: Beitrag in einer Fachzeitschrift › Artikel

  Open Access

  • Guanosine Diphosphate Mannose
  • Hydro-Lyases
  • Dehydration
  • Sugars
  • Deoxy Sugars
• 2015

  Process for the mono- and bi-​sialylation of glycoproteins employing N-​terminally truncated β-​galactoside α-​2,​6-​sialyltransferase mutants

  Czabany, T., Engel, A., Greif, M., Jung, C., Luley, C., Malik, S., Mueller, R., Nidetzky, B., Ribitsch, D., Schmoelzer, K., Schwab, H., Sobek, H., Suppmann, B., Thomann, M. & Zitzenbacher, S., 8 Jan 2015, Patent Nr. WO 2015001033 A1

  Publikation: Schutzrecht › Patent
• 2013

  Synergistic Effects Among Cellulases during Enzymatic Cellulose Degradation, Visualized by In-situ Atomic Force Microscopy

  Ganner, T., Bubner, P., Eibinger, M., Mayrhofer, C., Plank, H. & Nidetzky, B., 2013, Life Sciences; Multimodal and Interdisciplinary Microscopies. ., S. 525-526

  Publikation: Beitrag in Buch/Bericht/Konferenzband › Beitrag in einem Konferenzband