Bernd Nidetzky

Univ.-Prof. Dipl.-Ing. Dr.techn.

1966 …2022
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Forschungsoutput 1966 2019

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2019

Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, in : Catalysis Science & Technology. 9, 3, S. 842-853 12 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Open Access
Nitriles
Proton transfer
Catalysis
Escherichia coli
Protons
2018

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Okt 2018, in : Biotechnology and Bioengineering. 115, 10, S. 2416-2425 10 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Glucose 1-Dehydrogenase
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Bacillus megaterium
Glucose

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisán, J. M. & Nidetzky, B., 28 Feb 2018, in : ACS Applied Materials & Interfaces. 10, 8, S. 6858-6868 11 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Optical sensors
Proteins
pH sensors
Penicillin Amidase
Immobilized Enzymes

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mär 2018, in : ChemCatChem. 10, 5, S. 1192-1201

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

decarboxylation
Carboxy-Lyases
fatty acids
Hydrocarbons
Fatty acids

Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi

Rother, C., Gutmann, A., Gudiminchi, R., Weber, H., Lepak, A. & Nidetzky, B., 16 Mär 2018, in : ChemBioChem. 19, 6, S. 596-603 8 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Lipomyces
Glucose-6-Phosphate
Phosphotransferases
Adenosine Diphosphate
Adenosine Triphosphate

Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors

Bolivar, J. M., Valikhani, D. & Nidetzky, B., 9 Aug 2018, in : Biotechnology Journal.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Immobilized Enzymes
sucrose phosphorylase
Enzymes
Biocatalysis
Biotransformation

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

Jung, J. & Nidetzky, B., 1 Jan 2018, in : Journal of Biological Chemistry. 293, 10, S. 3720-3733 14 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Nitriles
Imines
Escherichia coli
Amines
Oxidoreductases

Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Schmölzer, K., Lemmerer, M. & Nidetzky, B., 1 Mär 2018, in : Biotechnology and Bioengineering. 115, 3, S. 545-556 12 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Glycosyltransferases
Glucosides
Polyphenols
Phloretin
Glycosylation

l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: Aeration and carbon source influence yields and productivities

Novy, V., Brunner, B. & Nidetzky, B., 11 Apr 2018, in : Microbial cell factories . 17, 1, 59.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Xylose
Lactic acid
Yeast
Glucose
Saccharomyces cerevisiae
Cellulose 1,4-beta-Cellobiosidase
Cellulose
Hydrolysis
Cellulases
Enzymes

New flavanol O-glycosides in grape and wine

Zerbib, M., Mazauric, J. P., Meudec, E., Le Guernevé, C., Lepak, A., Nidetzky, B., Cheynier, V., Terrier, N. & Saucier, C., 15 Nov 2018, in : Food chemistry. 266, S. 441-448 8 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Wine
Catechin
Vitis
flavanols
Glycosides

Process intensification for O2-dependent enzymatic transformations in continuous single-phase pressurized flow

Bolivar, J. M., Mannsberger, A., Thomsen, M. S., Tekautz, G. & Nidetzky, B., 4 Dez 2018, in : Biotechnology and Bioengineering.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Oxidoreductases
Biotransformation
Catalase
Pressure
Glucose Oxidase

Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

Benítez-Mateos, A. I., Nidetzky, B., Bolivar, J. M. & López-Gallego, F., 21 Feb 2018, in : ChemCatChem. 10, 4, S. 654-665 12 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Biocatalysts
Immobilized Enzymes
Enzymes
enzymes
Structural integrity

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Escobar, S., Bernal, C., Bolivar, J. M., Nidetzky, B., López-Gallego, F. & Mesa, M., 1 Apr 2018, in : Molecular Catalysis. S. 106-113 8 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Polyethyleneimine
Lipases
Lipase
Encapsulation
Silicon Dioxide

β-Glucosyl Fluoride as Reverse Reaction Donor Substrate and Mechanistic Probe of Inverting Sugar Nucleotide-Dependent Glycosyltransferases

Lepak, A., Gutmann, A. & Nidetzky, B., 5 Okt 2018, in : ACS Catalysis. 8, 10, S. 9148-9153 6 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Flavonoids
Glycosyltransferases
Diphosphates
Uridine
Nucleotides
2017

An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, in : Organic & biomolecular chemistry. 15, S. 7917 7924 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Okt 2017, in : ACS Applied Materials & Interfaces. 9, 40, S. 34641-34649 9 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Enzyme immobilization
Microfluidics
Silicon Dioxide
Enzymes
Silica

Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, in : Advanced Synthesis & Catalysis. 359, S. 292-301

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Open Access
Sugar (sucrose)
Nucleotides
Nucleosides
Glucose
Diphosphates

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, in : Biotechnology Journal.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Uridine Diphosphate Glucose
Glycosyltransferases
Uridine Diphosphate
Recycling
Biological Products

Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

Leis, D., Lauß, B., Macher-Ambrosch, R., Andreas, P., Nidetzky, B. & Kratzer, R., 2017, in : Journal of Biotechnology. 257, S. 110-117

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, in : Angewandte Chemie / International Edition . 56, S. 2503 –2507

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Xylose
Uridine Diphosphate
Isotopes
Enzymes
Sugars

Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High-Performance Biocatalytic Microreactor

Valikhani, D., Bolivar, J. M., Pfeiffer, M. & Nidetzky, B., 9 Jan 2017, in : ChemCatChem. 9, 1, S. 161-166 6 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

sucrose phosphorylase
sucrose
Sugar (sucrose)
microchannels
Microchannels

Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

Bolivar, J. M., Luley-Goedl, C., Leitner, E., Sawangwan, T. & Nidetzky, B., 10 Sep 2017, in : Journal of Biotechnology. 257, S. 131-138 8 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

sucrose phosphorylase
Sugar (sucrose)
Glycerol
Catalyst supports
Enzymes

Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, in : Angewandte Chemie. 129, S. 2544 –2548

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

Diricks, M., Gutmann, A., Debacker, S., Dewitte, G., Nidetzky, B. & Desmet, T., 2017, in : Protein engineering, design and selection. 30, 3, S. 141-148

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Uridine Diphosphate
Sugar (sucrose)
Nucleotides
Administrative data processing
Diphosphates

Single-molecule study of oxidative enzymaticdeconstruction of cellulose

Eibinger, M., Sattelkow, J., Ganner, T., Plank, H. & Nidetzky, B., 2017, in : Nature Communications . S. 8:894 7 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschung

Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

Viefhues, M., Sun, S., Valikhani, D., Nidetzky, B., Vrouwe, E. X., Mayr, T. & Bolivar, J. M., 10 Mai 2017, in : Journal of Micromechanics and Microengineering. 27, 6, 065012.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Oxygen sensors
Immobilized Enzymes
Glucose Oxidase
Glucose oxidase
Optical sensors

Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, in : Biotechnology and Bioengineering. 114, 1, S. 163-171 9 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Xylose
L-Lactate Dehydrogenase
Yeast
Fermentation
Glucose

Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

Gudiminchi, R. K. & Nidetzky, B., 18 Jul 2017, in : ChemBioChem. 18, 14, S. 1387-1390 4 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

sucrose phosphorylase
Ascorbic Acid
Sucrose
Cosmetics
Protonation
2016

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Bolivar, J. M., Eisl, I. & Nidetzky, B., 1 Jan 2016, in : Catalysis today. 259, S. 66-80 15 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Immobilized Enzymes
Biocatalysts
Enzymes
Catalyst supports
Proteins
Fucosyltransferases
Oligosaccharides
Human Milk
galactoside 3-fucosyltransferase
Guanosine Diphosphate Fucose

Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

Eibinger, M., Zahel, T., Ganner, T., Plank, H. & Nidetzky, B., 8 Mär 2016, in : Biotechnology for Biofuels . 9, 56

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Development of a fully integrated falling film microreactor for gas-liquid-solid biotransformation with surface immobilized O2-dependent enzyme

Bolivar Bolivar, J. M., Krämer, C. E. M., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, in : Biotechnology and Bioengineering.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Biotransformation
Enzymes
Gases
Immobilization
Oxidoreductases

Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis

Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2 Sep 2016, in : Scientific reports. S. 1-11 11 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

Lemmerer, M., Schmoelzer, K., Gutmann, A. & Nidetzky, B., 2016, in : Advanced Synthesis & Catalysis. 358, S. 3113-3122

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Sugar (sucrose)
Nucleosides
Sugars
Chromatography
Anions

Functional characterization of thenative swollenin from Trichoderma reesei:study of its possible role as C1 factor ofenzymatic lignocellulose conversion

Eibinger, M., Sigl, K., Sattelkow, J., Ganner, T., Ramoni, J., Seiboth, B., Plank, H. & Nidetzky, B., 2016, in : Biotechnology for Biofuels . S. 9:178

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli

Jung, J., Czabany, T., Wilding, B., Klempier, N. & Nidetzky, B., 2016, in : The Journal of Biological Chemistry. 291, 49, S. 25411-25426 16 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Nitriles
Escherichia coli
Oxidoreductases
Kinetics
Substrates

Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

Bolivar Bolivar, J. M., Tribulato, M. A., Petrasek, Z. & Nidetzky, B., 1 Nov 2016, in : Biotechnology and Bioengineering. 113, 11, S. 2342-2349 8 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Enzyme immobilization
Immobilization
Glass
Amino acids
Oxidoreductases

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

Dewitte, G., Walmagh, M., Diricks, M., Lepak, A., Gutmann, A., Nidetzky, B. & Desmet, T., 1 Jun 2016, in : Journal of Biotechnology. 233, S. 49-55

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Stevia
Glycosylation
Glycosyltransferases
Uridine Diphosphate
Glycosides

Special Issue on acib, Dedicated to the Occasion of Prof. Dr. Helmut Schwab's 65th Birthday

Nidetzky, B. & Sensen, C. W., 14 Jul 2016, in : Journal of Biotechnology.

Publikation: Beitrag in einer FachzeitschriftArtikelForschung

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

Schmoelzer, K., Gutmann, A., Diricks, M., Desmet, T. & Nidetzky, B., 2016, in : Biotechnology Advances. 34, 2, S. 88-111

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Glycosylation
Glycosyltransferases
Diphosphates
Sugar (sucrose)
Nucleosides
Trichoderma
micromorphology
Cellulase
Lactose
Enzymes

Unlocking the Potential of Leloir Glycosyltransferases for Applied Biocatalysis: Efficient Synthesis of Uridine 5′-Diphosphate-Glucose by Sucrose Synthase

Gutmann, A. & Nidetzky, B., 25 Okt 2016, in : Advanced Synthesis & Catalysis. 358, S. 3600-3609

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Glycosyltransferases
Diphosphates
Uridine
Sugar (sucrose)
Glucose

β-Cyclodextrin Improves Solubility and Enzymatic C-Glucosylation of the Flavonoid Phloretin

Bungaruang, L., Gutmann, A. & Nidetzky, B., 2016, in : Advanced Synthesis & Catalysis. 358, 3, S. 486-493

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Phloretin
Flavonoids
Cyclodextrins
Solubility
Complexation
2015

All-in-one assay for β-D-galactoside sialyltransferases: quantification of productive turnover, error hydrolysis and site selectivity

Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D. & Nidetzky, B., 2015, in : Analytical biochemistry. 483, S. 47-53

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases.

Czabany, T., Bubner, P., Luley, C. & Nidetzky, B., 2015, in : Analytical biochemistry. 490, S. 46-51

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, in : Chemical communications. S. 3083-3086

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Creating a Water-Soluble Resveratrol-Based Antioxidant by Site-Selective Enzymatic Glucosylation

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 2015, in : ChemBioChem. 16, 13, S. 1870-1874

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Antioxidants
Water
Solubility
Glycosylation
Glucosyltransferases

Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

Wildberger, P., Pfeiffer, M., Brecker, L. & Nidetzky, B., 21 Dez 2015, in : Angewandte Chemie / International Edition . 54, 52, S. 15867-15871 5 S.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Phosphorylase Phosphatase
Phosphatases
Phosphates
Phosphorylation
Catalysts

Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts

Trausinger, G., Gruber, C., Krahulec, S., Magnes, C., Nidetzky, B. & Klimacek, M., 2015, in : Biotechnology for Biofuels . 8:157, S. 1-13

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung